3. We anticipate our recombinant DHFR protein to be functional for use in future research applications. However, if you had been able to test functionality and found that your recombinant GST-DHFR-His protein was not functional, what would you propose is one potential issue you would investigate to troubleshoot further. In other words, what is one thing that could have gone wrong to result in non-functional protein from the method used and results we obtained this semester? Please be specific with the issue, where in the procedure it would have occurred, and explain your rationale.

We anticipate our recombinant DHFR protein to be functional for use in future research applications. However, if you had been able to test functionality and found that your recombinant GST-DHFR-His protein was not functional, what would you propose is one potential issue you would investigate to troubleshoot further. In other words, what is one thing that could have gone wrong to result in non-functional protein from the method used and results we obtained this semester? Please be specific with the issue, where in the procedure it would have occurred, and explain your rationale.