Experimental Hypoxia

Healthcare&Medicine

Please read both PPT and manual carefully. Most of the answers can be found in the PPT Slides and Experiment Manual. Mice survival time can be found at the end PPT Slides. However, if you can’t find the information, you may search on the internet. No word limit but you can write around 100 words for each question. Answers must have medical/scientific evidence to support your argument. References no needed.

Questions
1. BLANK – THERE IS NO QUESTION 1
2. In each hypoxia model, what is the difference in the color of mouse’s tail, ears, lip, mucosa, and blood? Why Medically?
3. In the hypotonic hypoxia experiment, is there any difference in the survival time of the two mice and the content of oxygen and carbon dioxide in the respective hypoxic bottles? Why Medically? (Hint: Soda Lime – Absorb Carbon Dioxide. Glass Beads – Mice will exhale carbon dioxide – Prevents Carbon Dioxide Poisoning)
4. In the sodium nitrite poisoning hypoxia experiment, is there any difference in survival time between the two mice? Why Medically? (Hint: Methylene Blue – Ferrous Ion to Ferric Ion. Saline – mixture of sodium chloride and water)
5. What might be the difference in the response of neonatal and adult mice to a decrease in PaO2 in inhaled air? Why Medically? (Hint: Anesthetics – Metabolic activity much lower. Normal Adult – inhale more oxygen. Why baby mouse is more tolerance to hypoxia?)
6. What is the difference in the hypoxia tolerance of mice at different temperature (low temperature, high temperature)? Why Medically? (Hint: Cold/Low Temp. Survive much longer).
7. What is the difference between hypoxia tolerance in awake and anesthetized mice? Why Medically?