What function does your protein provide in S. pyogenes? Why do you think it will make a good GAS vaccine candidate?
PART 1
You have an idea for a vaccine to prevent Group A Strep (GAS) infections. You know that Streptococcus bacteria are fastidious organisms, and you don’t expect to be able to produce your protein of interest in large quantities in S. pyogenes. Based on your knowledge of GAS, design a recombinant vaccine candidate to protect against GAS infection using E. coli as your recombinant protein production organism. Your choice of GAS antigen should be something produced by GAS that is not produced by humans. Your GAS protein(s) of choice will be expressed in E. coli and then purified for use in vaccine production. (Yes, I am expecting you to find a GAS antigen on your own.) To express your protein in E. coli, you need to clone the gene(s) of interest into a plasmid under the control of the Plac promoter. Create and upload a diagram that shows each step of your design strategy and cloning process. Start with getting your gene of interest out of S. pyogenes and end with your selection and screening process in E. coli. Be sure to include all of the following:
– What GAS protein(s) will be expressed by your recombinant E. coli?
– Show all components that need to be present on your plasmid for replication, selection, screening, and for regulation by the Plac promoter.
– How do all of the parts come together?
– A selection mechanism to ensure that only recombinant E. coli expressing your plasmid can grow. Any selection mechanism is ok. Indicate what media needs to be used and what you expect to see.
– A screening mechanism to show that your gene(s) of interest is being expressed. Indicate what media needs to be used and what you expect to see.
PART 2
1. What S. pyogenes protein(s) are you expressing in E. coli for your recombinant vaccine candidate?
2. What function does your protein provide in S. pyogenes? Why do you think it will make a good GAS vaccine candidate?
3. Are there any concerns about negative side effects or potential toxicity based on the GAS protein you have chosen? Would you expect your protein to cause an infection or disease symptoms in the host or have some other negative impact? Justify your answer.
4. If your protein of interest proves to be only mildly immunogenic when tested, what could you do to improve its immunogenicity? (1-2 sentences is fine)
5. Do you expect to see a cellular or humoral immune response to your vaccine? What part of the immune response could you measure to determine whether or not the vaccine is effective?
6. What advantage(s) does your vaccine have compared to a live GAS vaccine? Provide at least one advantage.
7. What disadvantage(s) does your vaccine have compared to a live GAS vaccine? Provide at least one disadvantage.
8. What media component(s) are necessary to ensure that your gene of interest is expressed in the recombinant E. coli cells?
9. What media component(s) would you need to add to shut down the expression of your gene in the recombinant E. coli cells?(I’ve attached my previous diagram of recombinant DNA)
How is data analytics different from statistics?
How is data analytics different from statistics?
Analytics tools fall into 3 categories: descriptive, predictive, and prescriptive. What are the main differences among these categories?
Explain how businesses use analytics to convert raw operational data into actionable information. Provide at least 1 example.
Consider the organization you work for (or another organization you’re familiar with). Does this organization use data analytics? If so, how is it used? If not, how could the organization use data analytics to improve its performance?
What is the difference between individual racism and systemic racism?
Consider the case of the person who stole medicine he could not afford in order to ease the pain of his suffering child. How would Kant judge the act? How would Mill judge the act? What would Einstein say?
Why does Rand believe that capitalism serves human flourishing? Why does Marx believe that capitalism diminishes human flourishing? What does Einstein argue?
What is the difference between individual racism and systemic racism? How does King argue we need to address the issue? How does Malcolm X argue we need to address the issue? How would Einstein view this debate?
What is philosophy? Was Einstein a philosopher? Are you a philosopher?
Describe an event related to diversity that either you or someone around you has experienced. What did you take away, and how did it enhance your understanding of diversity?
Describe an event related to diversity that either you or someone around you has experienced. What did you take away, and how did it enhance your understanding of diversity?
Share a current issue related to diversity that you have found interesting or that has personally impacted you. Post an article from a news source. How does diversity help you better understand this issue?
Post at least two responses to your fellow students. In your responses to your peers, take a look through the lens of intersectionality and consider both sides of your classmates’ issues. Compare and contrast your answers. What do you like about your peers’ explanations, and what would you add?
Explain how your personal experiences and worldview might influence your discretion as a criminal justice professional working on this case.
Select one of the following cases:
Matthew Shepard
Charleston church shooting
Private Barry Winchell
Yusor Mohammad Abu-Salha, Deah Shaddy Barakat, and Razan Mohammad Abu-Salha
Review the Learning Resources related to your selected case.
Describe your thoughts and feelings about the case you selected. Explain how your personal experiences and worldview might influence your discretion as a criminal justice professional working on this case.
Discuss the Role of highly educated foreign human capital in the economic diversification of the United Arab Emirates
Discuss the Role of highly educated foreign human capital in the economic diversification of the United Arab Emirates
Discuss why Keynes believed the development of financial markets could make the capitalist investment more unstable.
In the “State of Long-Term Expectations,” Keynes argued that “as the organization of investment market improves,” there was a growing risk for “the capital development of a country” to become “the by-product of the activities of a casino”. Based on your reading of section 3 and 5 of the chapter, discuss why Keynes believed the development of financial markets could make the capitalist investment more unstable. Why did Keynes anticipate the state to take “an ever greater responsibility” for directly organizing investment?
Are cybersecurity threats similar from system to system?
A cybersecurity plan is a plan designed to protect an IT system.
Research some common cybersecurity threats to consider incorporating into the cybersecurity plan you will create for your project.
Write a 150-word response that addresses the following questions:
Are cybersecurity threats similar from system to system?
Where can you locate best practices for preventing or mitigating cybersecurity threats?
Write a paper in which you discuss the various methods that you can use to convert numbers between number bases.
Assignment Instructions
Write a paper in which you discuss the various methods that you can use to convert numbers between number bases. Be sure to include a discussion of at least three different conversion methods and provide a conversion example for each method that you explain. You should discuss both converting from base 10 to another type of number base, as well as converting another type of number base to base 10. Include binary to decimal and binary to hexadecimal as two of your conversion examples.
In a final paragraph, explain why it is important that you, as an IT professional, understand numeric conversion methods.
Assignment Requirements
Your paper should be 2–3 pages in length. Be sure to use appropriate APA format and cite your textbook or other sources that you used in your paper.
The paper should contain sufficient information to adequately answer the questions and contain no spelling, grammar, or APA errors.
Based on the four points of the diamond, are firms likely to be successful?
Select a country and industry and apply Porter’s diamond of national advantage framework to the industry and the country you have chosen. Based on the four points of the diamond, are firms likely to be successful? Is there any chance of governmental variables at play in your industry or country? If so, how might they improve or detract from national advantage?
Prepare a PowerPoint presentation of 8 slides summarizing your findings. Be sure to use graphics or illustrations to enhance your presentation. Visual interest and professionalism should be taken into consideration as you present the information.
Add additional content in the Notes section of the presentation.
Support your responses with examples.
