Use the DHFR fusion protein nucleotide sequence provided below. Hint: the DHFR fusion protein sequence you have been provided with here only contains one tag.
- Which reading frame (5’3’) did you use to translate the sequence: 1, 2, or 3? _______ (2pt)
- Where is the tag located: At the N-terminus or C-terminus of DHFR? ____________ (2pt)
- Briefly describe how you found the tag location in (b.) above. (2pt)
- What is the tag used: Myc, FLAG, GST, or His? _____________ (2pt)
What is the calculated molecular weight of this fusion protein? __________
